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xk 50 100 column  (Cytiva Europe)


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    Structured Review

    Cytiva Europe xk 50 100 column
    Xk 50 100 Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xk+50+100+column/XK+50%2F100+Column/us12612433-345-32-39
    Average 94 stars, based on 18 article reviews
    xk 50 100 column - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Fast Protein Liquid Chromatography:

    Article Title: Hemoglobin induced cell trauma indirectly influences endothelial TLR9 activity resulting in pulmonary vascular smooth muscle cell activation
    Article Snippet: .. Catalase and superoxide dismutase were removed using an AKTA FPLC system and an XK 16/70 column containing Superdex 200 medium or an XK 50/100 column containing Sephacryl 200 medium (GE Healthcare Bio-Sciences, Piscataway, NJ, USE) as previously described[ ]. .. Several runs were pooled and concentrated to 200 mg/mL using Centricon Plus– 70 with 30 kDa cutoff membrane filters (Millipore, Bilerica MA, USA).

    Article Title: Hemoglobin induced cell trauma indirectly influences endothelial TLR9 activity resulting in pulmonary vascular smooth muscle cell activation.
    Article Snippet: .. Catalase and superoxide dismutase were removed using an AKTA FPLC system and an XK 16/70 column containing Superdex 200 medium or an XK 50/100 column containing Sephacryl 200 medium (GE Healthcare BioSciences, Piscataway, NJ, USE) as previously described[13]. .. Several runs were pooled and concentrated to 200 mg/mL using Centricon Plus– 70 with 30 kDa cutoff membrane filters (Millipore, Bilerica MA, USA).

    other:

    Article Title: Large-scale preparation of β-1,2-glucan using quite a small amount of sophorose.
    Article Snippet: A large amount of β-1,2-glucan was produced enzymatically from quite a small amount of sophorose as an acceptor material through three synthesis steps using a sucrose phosphorylase and a 1,2-β-oligoglucan phosphorylase.. The first synthesis step was performed in a 200 μL of a reaction solution containing 5 mM sophorose and 1.0 M sucrose. β1,2-Glucan in a part of the resultant solution was hydrolyzed to β-1,2-glucooligosaccharides by a β-1,2-glucanase.. The second synthesis was performed in 25 times the volume for the first synthesis.

    Purification:

    Article Title: Identification of novel protein domain for sialyloligosaccharide binding essential to Mycoplasma mobile gliding
    Article Snippet: .. Purified rFoot was transferred into a dialysis tube and put into an empty XK 50/100 column (GE Healthcare) filled with 250 ml of 10 mM Tris–HCl pH 8.8 containing 4 M urea and 100 mM arginine ( available online). ..

    Sonication:

    Article Title: Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry
    Article Snippet: .. After sonication and ammonium sulfate precipitation, the precipitated protein pellet was resuspended in 40 mL 50 mM NaHCO 3 pH 9, 2 mM dithiothreitol (DTT) (Buffer X), then loaded onto a hand packed XK 50/100 column with Sephacryl S300 resin (GE Healthcare) and eluted overnight with Buffer X. Heterodimer Cp149 His6 Cp149 Y132A is supposed to be an assembly-incompetent dimer with the Y132A mutation and the two monomers, Cp149 His6 and Cp149 Y132A , and should have 1 to 1 ration. ..

    Article Title: Hepadnavirus capsid protein heterodimers and virus-like particles
    Article Snippet: .. After sonication and ammonium sulfate (AS) precipitation, the precipitated protein pellet was resuspended in 40 mL 50 mM NaHCO3 pH 9, 2 mM DTT (Buffer X), then loaded onto a hand packed XK 50/100 column with Sephacryl S300 resin (GE Healthcare) and eluted overnight with Buffer X. Heterodimer Cp149His6Cp149Y132A is supposed to be an assembly incompetent dimer, with the Y132A mutation and the two monomers, Cp149His6 and Cp149Y132A, and should have 1 to 1 ration. ..

    Mutagenesis:

    Article Title: Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry
    Article Snippet: .. After sonication and ammonium sulfate precipitation, the precipitated protein pellet was resuspended in 40 mL 50 mM NaHCO 3 pH 9, 2 mM dithiothreitol (DTT) (Buffer X), then loaded onto a hand packed XK 50/100 column with Sephacryl S300 resin (GE Healthcare) and eluted overnight with Buffer X. Heterodimer Cp149 His6 Cp149 Y132A is supposed to be an assembly-incompetent dimer with the Y132A mutation and the two monomers, Cp149 His6 and Cp149 Y132A , and should have 1 to 1 ration. ..

    Article Title: Hepadnavirus capsid protein heterodimers and virus-like particles
    Article Snippet: .. After sonication and ammonium sulfate (AS) precipitation, the precipitated protein pellet was resuspended in 40 mL 50 mM NaHCO3 pH 9, 2 mM DTT (Buffer X), then loaded onto a hand packed XK 50/100 column with Sephacryl S300 resin (GE Healthcare) and eluted overnight with Buffer X. Heterodimer Cp149His6Cp149Y132A is supposed to be an assembly incompetent dimer, with the Y132A mutation and the two monomers, Cp149His6 and Cp149Y132A, and should have 1 to 1 ration. ..

    Chromatography:

    Article Title: B-cell reduction using CD37-specific and CD20-specific binding molecules
    Article Snippet: .. Using dPBS, an XK 50/100 column (GE healthcare XK 50/100 empty chromatography column, Catalog #18-8753-01) containing 1 L of Superdex 200 FF sepharose was equilibrated at 12.6 mls/min (38 cm/hr) for 1.5 column volumes (CV). ..



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